Journal: Brazilian Journal of Medical and Biological Research

Article Title: A method to assess heart rate variability in neonate rats: validation in normotensive and hypertensive animals

doi: 10.1590/1414-431X20209493

Figure Lengend Snippet: A , Electrocardiographic (ECG) data acquisition in a neonate rat. B , ECG wave signals. C , Tachogram obtained by cardiac interval recording.

Article Snippet: RR intervals were recorded over a 30-min period using an ECG system (BioAmp FE231, ADInstruments Ltd.) and LabChart software (version 8, ADInstruments Ltd.).

Techniques:

Journal: bioRxiv

Article Title: Organ-specific translation elongation rates measured by in vivo ribosome profiling

doi: 10.1101/279257

Figure Lengend Snippet: Experimental setup. (A) Layout of mouse organs susceptible to translation inhibitors. Organs marked in green are permeable for both harringtonine and cy-cloheximide within 15 sec post-injection. Translation elongation rate can be directly measured in these organs. Testes (shown in yellow) are partially permeable, and brain is mostly unaffected by inhibitors. (B) High doses of harringtonine severely drop heartbeat rate. We established that 2.5 mg/ml had no effect on the beat rate, whereas 5 mg/ml decreased it twice. 10 mg/ml caused cardiac arrest in most of the cases. All injections were done in a tail vein - injection volume was 200 μ l and the weight of mice was 20-25 g. (C) Representative electrocardiograms (ECG) from injected mice. The upper track shows the slowdown of the beat rate in response to 5 mg/ml harringtonine. The lower track shows no difference after the injection of 2.5 mg/ml harringtonine. The effect of harringtonine appears roughly 20 sec after the injection. The red line marks the injection event.

Article Snippet: Heart function was monitored by ECG (AD Instruments, Powerlab 8/30 recorder with BioAmp electrodes).

Techniques: Injection

Journal: bioRxiv

Article Title: Organ-specific translation elongation rates measured by in vivo ribosome profiling

doi: 10.1101/279257

Figure Lengend Snippet: Measuring translation elongation rate in vivo . (A) ECG monitoring of heart function before and after drug injection to ensure the heart beat rate is unaffected at the chosen dosage. (B) Experimental design for a time-resolved study. (C) Linear regression analysis of injection timepoints to infer the elongation rate. (D) Comparison of mean translation elongation rates in three organs. (E) Metagene analysis of ribosome profiling sequences. Genes longer than 2,000 nucleotides are presented. The first 1,500 nucleotides are shown starting from the first codon of the reading frame. Solid lines represent mean signal from biological replicates, and light shading corresponds to a standard deviation.

Article Snippet: Heart function was monitored by ECG (AD Instruments, Powerlab 8/30 recorder with BioAmp electrodes).

Techniques: In Vivo, Injection, Comparison, Standard Deviation

Journal: Frontiers in Cell and Developmental Biology

Article Title: Forward genetic screen using a gene-breaking trap approach identifies a novel role of grin2bb -associated RNA transcript ( grin2bbART ) in zebrafish heart function

doi: 10.3389/fcell.2024.1339292

Figure Lengend Snippet: The electrocardiogram profile of bigheart fish indicates signs of arrhythmia. (A) Electrocardiogram recordings of wild-type ( bh +/+ ) zebrafish. X-axis represents time in milliseconds (ms), and Y-axis represents voltage in millivolts (mV). (B) Electrocardiogram recording of bh −/− fish, red arrowhead represents the absence of beats. (C) Graph representing the R-R interval in wild type and bh −/− fish. (D) Graph representing heart beat rate in adult wild type ( bh +/+ ) zebrafish and bh −/− fish. (E) Graph showing an overlay of wild type and bh −/− mutant ECG recordings (Regular line and dashed line represents the ECG profiles of wild type and bh −/− fish, respectively). P, Q, R, S and T represent regular ECG waves. (**, p < 0.01). The bars indicate the average values ± SD.

Article Snippet: ECG recordings were obtained by inserting two needle electrodes (AD Instruments, Animal Bioamp) through the ventral epidermis.

Techniques: Mutagenesis